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3D Assays: Intelligent Software, Insightful Analyses

3D Assays: Intelligent Software, Insightful Analyses

In this webinar, we discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

Presenters:

Daniel Bemmerl, Area Manager, High-End Microscopy Systems
Shohei Imamura, Strategic Project Manager, Regenerative Medicine and Drug Discovery Solutions

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FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


相关产品

3D细胞分析软件

NoviSight

  • 从完整结构到亚细胞级别的3D图像识别
  • 统计分析
  • 多种默认检测方法

3D Assays: Intelligent Software, Insightful Analyses

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Webinar: 3D Assays: Intelligent Software, Insightful Analyses

In this webinar, our experts discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


相关产品

3D细胞分析软件

NoviSight

  • 从完整结构到亚细胞级别的3D图像识别
  • 统计分析
  • 多种默认检测方法
Experts
Daniel Bemmerl
高端显微镜系统的区域经理

我叫Daniel Bemmerl,我可以帮助您了解3D高内涵分析和类器官成像相关的信息。我加入奥林巴斯时担任TIRF显微镜和高内涵筛选的应用专家,目前则专注于3D分析软件。

我曾经从事分子和发育干细胞生物学研究,之后很快意识到相对于处理标本,我对研究的技术层面,特别是成像本身更加感兴趣。之所以加入奥林巴斯生命科学部门,是因为我更喜欢实验室之外的工作,成为沟通客户与开发部门的桥梁。我非常乐意回答您有关3D成像的任何问题,并帮助您改善成像工作流程,让您能够专注从事研究工作。

Shohei Imamura
战略项目经理

我叫Imamura Shohei,是奥林巴斯的战略项目经理。我在科学显微镜的销售方面拥有4年经验,并在侧重软件的产品规划方面拥有7年经验。我还负责战略项目管理和执行。我拥有日本明治大学的商业学士学位。

3D Assays: Intelligent Software, Insightful Analyses2021年10月18日
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